|
KMID : 0545120170270010092
|
|
Journal of Microbiology and Biotechnology
2017 Volume.27 No. 1 p.92 ~ p.100
|
|
|
Efficient (3R)-Acetoin Production from meso-2,3-Butanediol Using a New Whole-Cell Biocatalyst with Co-Expression of meso-2,3-Butanediol Dehydrogenase, NADH Oxidase, and Vitreoscilla Hemoglobin
|
|
Guo Zewang
Zhao Xihua
He Yuanzhi
Yang Tianxing
Gao Huifang
Li Ganxin
Chen Feixue
Sun Meijing
Lee Jung-Kul
Zhang Liaoyuan
|
|
|
Abstract
|
|
|
|
Acetoin (AC) is a volatile platform compound with various potential industrial applications. AC contains two stereoisomeric forms: (3S)-AC and (3R)-AC. Optically pure AC is an important potential intermediate and widely used as a precursor to synthesize novel optically active materials. In this study, chiral (3R)-AC production from meso-2,3-butanediol (meso-2,3- BD) was obtained using recombinant Escherichia coli cells co-expressing meso-2,3-butanediol dehydrogenase (meso-2,3-BDH), NADH oxidase (NOX), and hemoglobin protein (VHB) from Serratia sp. T241, Lactobacillus brevis, and Vitreoscilla, respectively. The new biocatalyst of E. coli/pET-mbdh-nox-vgb was developed and the bioconversion conditions were optimized. Under the optimal conditions, 86.74 g/l of (3R)-AC with the productivity of 3.61 g/l/h and the stereoisomeric purity of 97.89% was achieved from 93.73 g/l meso-2,3-BD using the whole-cell biocatalyst. The yield and productivity were new records for (3R)-AC production. The results exhibit the industrial potential for (3R)-AC production via whole-cell biocatalysis.
|
|
|
KEYWORD
|
|
|
meso-2, 3-butanediol dehydrogenase, meso-2, 3-butanediol, (3R)-acetoin, NAD+ regeneration, vitreoscilla hemoglobin, whole-cell biocatalysis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|